Dosage protocol · Experimental Research
PNC-27 30 mg
Typical daily range: 100–500 mcg once daily (gradual titration).
- Reconstitute: Add 3.0 mL bacteriostatic water → 10 mg/mL concentration.
- Typical daily range: 100–500 mcg once daily (gradual titration).
- Easy measuring: At 10 mg/mL, 1 unit = 0.01 mL = 100 mcg on a U‑100 insulin syringe.
- Storage: Lyophilized: freeze at −20 °C (−4 °F); after reconstitution, refrigerate at 2–8 °C (35.6–46.4 °F); avoid freeze–thaw cycles.
BAC water3.0mL
Concentration10mg/mL
Per unit (0.01 mL)100mcg
Reconstitution
PNC-27 dosage chart
PNC-27 dosage chart
| Week | Dose | Draw |
|---|---|---|
| Weeks 1–2 | 100 mcg0.10 mg | 1 unit0.01 mL |
| Weeks 3–4 | 200 mcg0.20 mg | 2 units0.02 mL |
| Weeks 5–8 | 300 mcg0.30 mg | 3 units0.03 mL |
| Weeks 9–12 | 400 mcg0.40 mg | 4 units0.04 mL |
| Weeks 13–16 | 500 mcg0.50 mg | 5 units0.05 mL |
How much to draw
The Draw column shows exactly where to stop on a U-100 insulin syringe. Different vial size or dose? The calculator below recomputes it live.
Supplies needed
Supplies needed
Peptide vial
The lyophilized compound
Bacteriostatic water
For reconstitution
U-100 insulin syringes
One per injection
Alcohol swabs
Stopper + site each time
How it works
PNC-27 is a synthetic, p53-derived research peptide described by the U.S. National Cancer Institute's Drug Dictionary as having potential antineoplastic activity.[1] It combines a fragment of the p53 tumor-suppressor protein that can bind the regulatory protein HDM-2 (also called MDM2) with a separate membrane residency segment designed to anchor the peptide at cell membranes.
PNC-27 is strictly an investigational compound studied in laboratory and animal cancer models. It is not an approved cancer drug, and it should never be treated as a substitute for oncology care.
Benefits & side effects
Published PNC-27 research is limited to preclinical work: cytotoxicity experiments in cancer cell lines including pancreatic, breast, and leukemia cells, comparisons with a designated negative-control peptide to test mechanism specificity, and some animal xenograft studies using human tumor cells implanted in immunodeficient mice.[2] [3]
These experiments report that PNC-27 reduces cancer cell viability and causes membrane damage under laboratory conditions, with some studies describing apparent selectivity for cancer over normal cells in culture. No human clinical trials of PNC-27 have been identified in public trial registries, and no peer-reviewed evidence establishes tumor shrinkage, survival benefit, or any patient-level outcome.[16] Laboratory cytotoxicity is a research signal, not proof of a working cancer treatment.
Calculator
Plan your own dose
Reconstituting a different vial size or targeting a different dose? Run the numbers.
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