Dosage protocol · Hormonal & Sexual Health
Oxytocin 10 mg
Typical daily range: 100–500 mcg once daily (gradual titration).
- Reconstitute: Add 3.0 mL bacteriostatic water → ~3.33 mg/mL concentration.
- Typical daily range: 100–500 mcg once daily (gradual titration).
- Easy measuring: At 3.33 mg/mL, 1 unit = 0.01 mL ≈ 33.3 mcg on a U‑100 insulin syringe.
- Storage: Lyophilized: freeze at −20 °C (−4 °F) or refrigerate at 2–8 °C (35.6–46.4 °F); after reconstitution, refrigerate at 2–8 °C (35.6–46.4 °F) for up to 28–30 days.
BAC water3.0mL
Concentration3.33mg/mL
Reconstitution
Oxytocin dosage chart
Oxytocin dosage chart
| Week | Dose | Draw |
|---|---|---|
| Weeks 1–2 | 100 mcg | 3 units0.03 mL |
| Weeks 3–4 | 200 mcg | 6 units0.06 mL |
| Weeks 5–6 | 300 mcg | 9 units0.09 mL |
| Weeks 7–8 | 400 mcg | 12 units0.12 mL |
| Weeks 9–12 | 500 mcg | 15 units0.15 mL |
How much to draw
The Draw column shows exactly where to stop on a U-100 insulin syringe. Different vial size or dose? The calculator below recomputes it live.
Supplies needed
Supplies needed
Peptide vial
The lyophilized compound
Bacteriostatic water
For reconstitution
U-100 insulin syringes
One per injection
Alcohol swabs
Stopper + site each time
How it works
Oxytocin is a naturally occurring cyclic nonapeptide hormone, produced in the hypothalamus and released into the bloodstream via the posterior pituitary gland.[1] As an endogenous neuropeptide it does double duty: peripherally, it drives uterine contraction and the milk-ejection reflex during breastfeeding, and centrally, it modulates brain circuits involved in social behavior and stress.[3]
Oxytocin occupies an unusual place among peptides covered on this site: synthetic oxytocin injection is an FDA-approved prescription drug for specific obstetric indications.[2] That approval applies only to the clinically administered injectable form. Oxytocin nasal sprays and other products marketed for bonding, trust, or mood are a separate, unapproved category and should not be assumed to carry the same evidence or regulatory standing.
Benefits & side effects
The strongest evidence for oxytocin is obstetric: it is an established uterotonic for medically indicated labor induction or augmentation and for preventing or treating postpartum hemorrhage, a role reflected in international guideline recommendations.[12]
Evidence for oxytocin's behavioral and psychiatric effects is far less settled. Early intranasal studies reported effects on trust behavior and amygdala responses to emotional faces, fueling popular interest, but larger, more rigorous trials have not held up that promise.[8] A 24-week randomized trial in children and adolescents with autism spectrum disorder found no significant benefit over placebo, and a meta-analysis of intranasal oxytocin for negative symptoms of schizophrenia found no consistent effect.[10] [11] Researchers in the field describe a need for better reproducibility and clearer dosing before any behavioral use could be considered established.
Calculator
Plan your own dose
Reconstituting a different vial size or targeting a different dose? Run the numbers.
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