Dosage protocol · Longevity & Mitochondrial Health
SS-31 50 mg
Typical daily range: 5–10 mg once daily (gradual titration); advanced protocols may reach 15–20 mg/day under supervision.
- Reconstitute: Add 3.0 mL bacteriostatic water → ~16.67 mg/mL concentration for convenient dosing.
- Typical daily range: 5–10 mg once daily (gradual titration); advanced protocols may reach 15–20 mg/day under supervision.
- Easy measuring: At 16.67 mg/mL, 1 unit = 0.01 mL ≈ 167 mcg on a U‑100 insulin syringe.
- Storage: Lyophilized: freeze at −20 °C (−4 °F); after reconstitution, refrigerate at 2–8 °C (35.6–46.4 °F); use within 4 weeks.
BAC water3.0mL
Concentration16.67mg/mL
Reconstitution
SS-31 dosage chart
SS-31 dosage chart · 1
| Week | Dose | Draw |
|---|---|---|
| Weeks 1–2 | 5 mg5000 mcg | 30 units0.30 mL |
| Weeks 3–8 | 10 mg10,000 mcg | 60 units0.60 mL |
SS-31 dosage chart · 2
| Week | Dose | Draw |
|---|---|---|
| Weeks 1–2 | 5 mg5000 mcg | 30 units0.30 mL |
| Weeks 3–4 | 10 mg10,000 mcg | 60 units0.60 mL |
| Weeks 5–8 | 15 mg15,000 mcg | 90 units0.90 mL |
| Optional Weeks 9–12 | 20 mg20,000 mcg | Split: 2 × 60 units0.60 mL |
How much to draw
The Draw column shows exactly where to stop on a U-100 insulin syringe. Different vial size or dose? The calculator below recomputes it live.
Supplies needed
Supplies needed
Peptide vial
The lyophilized compound
Bacteriostatic water
For reconstitution
U-100 insulin syringes
One per injection
Alcohol swabs
Stopper + site each time
How it works
SS-31 is a small, synthetic mitochondria-targeted tetrapeptide (four amino acids) that is better known by its clinical development name, elamipretide, along with earlier designations MTP-131 and Bendavia.[1] It belongs to the Szeto-Schiller family of aromatic-cationic peptides, originally designed to concentrate specifically within mitochondria rather than act at a cell-surface receptor.
Unlike many of the compounds covered on this site, elamipretide is a genuine investigational drug that has progressed into human clinical trials, including Phase 2 and Phase 3 studies, for specific rare mitochondrial diseases. That places it well ahead of unapproved research chemicals in terms of regulatory scrutiny, even though it is not yet a broadly FDA-approved medicine.
Benefits & side effects
Preclinical evidence is broad: mouse and cellular studies report improved mitochondrial respiration, reduced oxidative damage, and better skeletal-muscle bioenergetics in aged or injured tissue models.[4] Human evidence exists but is indication-specific and mixed. Clinical trials have evaluated elamipretide in primary mitochondrial myopathy (measuring walking distance and fatigue), Barth syndrome, heart failure, and ischemia-reperfusion settings, with results that vary by trial and endpoint rather than uniformly confirming benefit.[5]
The key distinction researchers draw is between biomarker improvement — such as better mitochondrial respiration readings — and clinical outcomes that matter to patients, like function, symptoms, or survival. For SS-31, the biomarker story is more consistent than the clinical-outcome story, and both should be read as disease-specific rather than generalizable to healthy adults seeking energy or anti-aging effects.
Calculator
Plan your own dose
Reconstituting a different vial size or targeting a different dose? Run the numbers.
1
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